The VIDAS is an enzyme-linked fluorescent immunoassay. Automated system is used for the detection of Campylobacter antigens using the ELFA technique (Enzyme Linked Fluorescent Assay). The Solid Phase Receptacle (SPR) serves as the solid phase as well as the pipetting device for the assay. The interior of the SPR is coated with anti-Campylobacter antibodies. The reagents for the assay are ready to use, and pre-dispensed in the sealed reagent strips. The sample is placed into the reagent strip by the user. Then the sample is cycled in and out of the SPR for a specific length of time. The unbound sample components are washed away. Alkaline phosphatade-labeled polylonal antibodies are cycled in, and out of the SPR and will bind to any Campylobacter antigen captured on the SPR wall. Further wash steps remove unbound conjugate. During the final detection step, the substrate is cycled in and out of the SPR. The conjugate enzyme catalyzes the hydrolysis of this substrate into a fluorescent product, the fluorescence of which is measured at 450nm. When the assay is completed, the results are analyzed automatically by the instrument, and a test value is generated for each sample. This value is then compared to an internal reference, and each result is interpreted (positive or negative). Turn around time is a minimum of four days.
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